Established (1995) by Aichun Dong, John F. Carpenter, and Winslow S. Caughey

All Rights Reserved


Solvent: 10 mM Potassium Phosphate/H2O, pH 7.3

Infrared Measurements:Nicolet 730, Magna 550, or Bomem-MB Fourier transform Infrared Spectrometer; Single beam mode with a 4 cm-1 resolution at room temperature.

Subtraction of Water Spectra:

Criterion I: The bands originating from water vapor must be subtracted accurately from the protein spectrum in the 1800 to 1500 cm-1 region regardless of the baseline (gaseous water subtraction).

Criterion II: A straight baseline must be obtained from 2000 to 1720 cm-1 (aqueous water subtraction).

Reference: Dong, A. & Caughey, W.S. (1994) Methods Enzymol. 232, 139-175.

Note:For affordable Replacement Infrared Windows, please contact Poudre Scientific Solution.


(1) All spectra are original difference spectra without smoothing. (2) The spectra are provided in both OMNIC (*.spa) and GRAMS (*.spc) formats. You must download spectral files first and then view and manipulate them with a proper software.
  • alpha1-Proteinase Inhibitor (Human)(SPA,SPC)
  • Albumin (Bovine serum, A-0281 Sigma)(SPA,SPC)
  • Albumin (Human serum)(SPA,SPC)
  • Alcohol Dehydrogenase (Equine Liver)(SPA,SPC)
  • Alcohol Dehydrogenase (Baker's Yeast)(SPA,SPC)
  • Basic Fibroblast Growth Factor (Recombinant, Human)(SPA,SPC)
  • Carbonic Anhydrase (Bovine Erythrocytes)(SPA,SPC)
  • Concanavalin A (Jack Bean)(SPA,SPC)
  • Alpha-Chymotrypsin (Bovine Pancreas)(SPA,SPC)
  • Alpha-Chymotrypsinogen A (Bovine Pancreas)(SPA,SPC)
  • Cytochrome c (Reduced; Equine Heart)(SPA,SPC)
  • Cytochrome c (Oxidized; Equine Heart)(SPA,SPC)
  • Cytochrome c (Oxidized; Bovine Heart)(SPA,SPC)
  • Cytochrome c (Oxidized; Tuna Heart)(SPA,SPC)
  • Cytochrome c (Oxidized; Baker's Yeast)(SPA,SPC)
  • Cytochrome c Oxidase (Oxidized; Bovine Heart)(SPA,SPC)
  • Deoxyribonuclease I (Bovine Pancreas)(SPA,SPC)
  • Elastase (Porcine Pancreas)(SPA,SPC)
  • Enolase (Baker's Yeast;3ENL)(SPA,SPC)
  • Factor XIII (Recombinant; Homodimer; Human)(SPA,SPC)
  • Ferritin (Apo, Horse Spleen)(SPA,SPC)
  • Ferritin (Fe-loaded, Horse Spleen)(SPA,SPC)
  • Fibrinogen (Human Plasma)(SPA,SPC)
  • Growth Hormone (Recombinant; Human)(SPA,SPC)
  • Hemoglobin (Carboxy; Human)(SPA,SPC)
  • Hemoglobin (Aquomet; Equine)(SPA,SPC)
  • Immunoglobulin G (Bovine)(SPA,SPC)
  • Interferon-gamma (Recombinant; Human)(SPA,SPC)
  • Alpha-Lactalbumin (Ca-depleted; Bovine Milk)(SPA,SPC)
  • Alpha-Lactalbumin (Ca-bound; Bovine Milk)(SPA,SPC)
  • Lactic Dehydrogenase (Rabbit Muscle)(SPA,SPC)
  • Beta-Lactoglobulin A (Bovine Milk)(SPA,SPC)
  • Beta-Lactoglobulin B (Bovine Milk)(SPA,SPC)
  • Holo-Lactoferrin (Human Milk)(SPA,SPC)
  • Light-chain LEN (Human recombinant; 2LVE)(SPA,SPC)
  • Lysozyme (Chicken Egg White)(SPA,SPC)
  • Myoglobin (Aquomet; Equine Heart)(SPA,SPC)
  • Ovalbumin (Chicken Egg)(SPA,SPC)
  • Papain (Papaya Latex)(SPA,SPC)
  • Poly-L-Lysine (pH7.4; Random Coil)(SPA,SPC)
  • Poly-L-Lysine (pH12.0; Heated; Beta-Sheet)(SPA,SPC)
  • RNase A (Bovine Pancreas)(SPA,SPC)
  • RNase S (Bovine Pancreas)(SPA,SPC)
  • Subtilisin Carlsberg (Bacillus licheniformis)(SPA,SPC)
  • Cu,Zn-Superoxide Dismutase (Oxidized; Bovine Liver)(SPA,SPC)
  • Cu,Zn-Superoxide Dismutase (Reduced; Bovine Liver)(SPA,SPC)
  • Staphylococcal Nuclease (Recombinant)(SPA,SPC)
  • Triosephosphate Isomerase (Rabbit Muscle)(SPA,SPC)
  • Trypsin (Bovine Pancreas)(SPA,SPC)
  • Trypsinogen (Bovine Pancreas)(SPA,SPC)
  • Trypsin Inhibitor (Soybean)(SPA,SPC)

If you have difficulties in retrieving spectral files, a disk with listed data files is available. Send your request via E-mail or write to:

Chemistry and Biochemistry Program
University of Northern Colorado
Greeley, CO 80639

Dong's Publications

DISCLAIMER: The content of the page is the sole responsibility of the author

URL FOR PAGE:http://www.unco.edu/nhs/chemistry/faculty/dong/irdata.htm

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