Aichun Dong, Ph.D.

Picture of Aichun Dong, Ph.D.

  • Professor
  • Department of Chemistry and Biochemistry
  • University of Northern Colorado
  • Greeley, CO 80639
  • Office: (970) 351-1284
  • Fax: (970) 351-2533
  • E-mail:

EDITOR-IN-CHIEF,International Journal of Biological Macromolecules



Yang, H.Y., Yang, S.-N., Kong, J.-L., Dong, A.*, & Yu, S.-N.*"Obtaining information about protein structures in aqueous solution using Fourier transform IR spectroscopy." Nature Protocols 10, 381-396, 2015.

Wu, G.-R., Gao, Z.-Y., Dong, A. and Yu, S.-N."Calcium-induced changes in calmodulin structural dynamics and thermodynamics." Intl. J. Biol. Macromol. 50, 1011-1017, 2012.[PDF]

Hoehne, M. Samuel, F.; Dong, A., Wurth, C.; Mahler, H.-C; Carpenter, J.F. and Randolph, T. "Adsorption of Monoclonal Antibodies to Glass Microparticles."J. Pharm. Sci. 100. 123-132,2010.[PDF]

Bai, S.-F.,and Dong A.* "Effects of immobilization onto aluminum hydroxide particles on the thermally induced conformational behavior of three model proteins." Intl. J. Biol. Macromol. 45, 80-85, 2009.[PDF]

Dutka, M.D., Dong, A., and Schwenz, R.W. "A Raman experiment exploring the definition of a strong acid. " Chem. Educator, 14, 16-18, 2009.[PDF]

Tyagi, A.K., Randolph, T.W., Dong, A., Maloney, K.M., Hitscherich Jr., C.,and Carpenter, J.F. "IgG particle formation during filling pump operation: A case study of heterogeneous nucleation on stainless steel nanoparticles." J. Pharm. Sci. 98, 94-109, 2009. [PDF]

Shen, X.-R., Li, H.-M., Ou, Y., Tao, W.-B., Dong, A., Kong, J.-L., Ji, C.-N., and Yu, S.-N. "The secondary structure of calcineurin regulatory region and conformational change induced by calcium/calmodulin binding." J. Biol. Chem. 283, 11407-11413, 2008. [PDF]

Dong, A.*, Jones, L.S., Kerwin, B.A., Krishnan, S., and Carpenter, J.F. "Secondary Structures of Proteins Adsorbed onto Aluminum Hydroxide Adjuvant: Infrared Spectroscopic Analysis of Proteins from Low Solution Concentrations." Anal. Biochem. 351, 282-289, 2006. [PDF]

Roy, S., Katayama, D., Dong, A., Kerwin, B.A., Randolph, T.W., and Carpenter, J.F. "Temperature Dependence of Benzyl Alcohol- and 8-Anilinonaphthalene-1-sulfonate-Induced Aggregation of Recombinant Human Interleukin-1 Receptor Antagonist." Biochemistry 45, 3898-3911, 2006. [PDF]

Dong, A.*, and Lam, T. "Equilibrium Titrations of Acid- Induced Unfolding-Refolding and Salt-Induced Molten Globule of Cytochrome c by FT-IR Spectroscopy." Arch. Biochem. Biophys. 436, 154-160, 2005. [PDF]

Madden, S.P., Wilson, W., Dong, A., Geiger, L., and Mecklin, C.J. "Multiple Linear Regression Using a Graphing Calculator-Applications in Biochemistry and Physical Chemistry.” J. Chem. Edu. 81, 903-907, 2004. [PDF]

Kim, Y.-S., Jones, L. S., Dong, A., Kendrick, B. S., Chang, B. S., Manning, M. C., Randolph, T. W., and Carpenter, J. F. "Effects of sucrose on conformational equilibria and fluctuations within native state ensembles of proteins.” Protein Sci. 12, 1252-1261, 2003. [PDF]

Huang, P., and Dong, A. "Thermal, chemical and chemothermal denaturation of yeast enolase." Spectroscopy 17, 453-467, 2003. [PDF]

Dong, A., Malecki, J., Lee, L., Carpenter, J.F. and Lee, J.C. "Ligand-induced conformational and structural dynamic changes in E. coil cyclic AMP receptor protein." Biochemistry 41, 6660-6667, 2002. [PDF]

Dong, A., Meyer, J.D., Manning, M.C., and Carpenter, J. F. "Comparative FTIR and CD spectroscopic analysis of alpha 1-proteinase inhibitor and ovalbumin in aqueous solution." Arch. Biochem. Biophys. 383, 148-155, 2000. [PDF]

Dong, A., Randolph, T., and Carpenter, J. F. "Entrapping intermediates of thermal aggregation in alpha-helical proteins with low concentration of guanidine hydrochloride." J. Biol. Chem. 275, 27689-27693, 2000. [PDF]

Yang, T., Dong, A., Meyer, J., Johnson, O.L., Cleland, J.L. and Carpenter, J.F. " Use of infrared spectroscopy to assess secondary structure of human growth hormone within biodegradable microspheres" J. Pharm. Sci. 88, 161-165, 1999. [PDF]

Hammack, B., Attfield, K., Clayton, D., Dec, E., Dong, A., Sarisky, C., and Bowler, B. "The magnitude of changes in guanidine-HCl unfolding m-values in the protein, iso-1-cytochrome c, depends upon the substructure containing the mutation." Protein Science 7, 1789-1795, 1998. [PDF]

Dong, A., Matsuura, J., Manning, M.C., and Carpenter, J.F. "Intermolecular beta-sheets from trifluoroethanol-induced non-native alpha-helical structure in beta-sheet predominant proteins: IR and CD spectroscopic study." Arch. Biochem. Biophys. 355, 275-281, 1998. [PDF]

Carpenter, J.F., Prestrelski, S.J.,and Dong, A. "Application of infrared spectroscopy to development of stable lyophilized protein formulations." Eur. J. Pharm. Biopharm. 45, 231-238, 1998. [PDF]

Yang, T., Dong, A., Meyer, J., Johnson, O.L., Carpenter, J.F., and Cleland, J.L. "Assessment of protein integrity within biodegradable microspheres" Proceed. Int’l Symp. Control. Rel. Bioact. Mater. 25, 257-258, 1998.

Dong, A., Kendrick, B., Kreilgaad, L., Matsuura, J., Manning, M.C., and Carpenter, J.F. "Spectroscopic study of secondary structure and thermal denaturation of recombinant human factor XIII in aqueous solution" Arch. Biochem. Biophys. 347, 213-220, 1997. [PDF]

Calvert, J. F., Hill, J. L., and Dong, A. "Redox-dependent conformational changes are common structural features of cytochrome c from various species" Arch. Biochem. Biophys. 346, 287-295, 1997. [PDF]

Stratton, L.P.,Dong, A., Manning, M.C., and Carpenter, J.F. " Drug delivery matrix containing native protein precipitate suspended in a poloxamer gel" J. Pharm. Sci. 86, 1006-1010, 1997. [PDF]

Dong, A., Kery, V., Matsuura, J., Manning, M.C., Kraus, J. P., and Carpenter, J.F. "Secondary structure of recombinant human cystathionine beta-synthase in aqueous solution: effect of ligand binding and proteolytic truncation" Arch. Biochem. Biophys. 344, 125-132, 1997. [PDF]

Godbole, S., Dong, A., Garbin, K., and Bowler, B.E. "A lysine 73 - histidine variant of yeast iso-1-cytochrome c: evidence for a native-like intermediate in the unfolding pathway and implications for m value effects" Biochemistry 36, 119-126, 1997. [PDF]

Dong, A., Meyer, J.D., Kendrick, B.S., Manning, M.C., and Carpenter, J.F. " Effect of secondary structure on the activity of enzyme suspended in organic solvents" Arch. Biochem. Biophys. 334, 406-414, 1996. [PDF]

Chang, B.S., Beauvais, R.M., Arakawa, T., Narhi, L.O., Dong, A., Aparisio, D.I., and Carpenter, J.F. "Formation of an active dimer during storage of interleukin-1 receptor antagonist in aqueous solution" Biophys. J. 71, 3399-3406, 1996. [PDF]

Dong, A.*, Hyslop, R.M., and Pringle, D.L. "Differences in conformational dynamics of RNase A and S as observed by infrared spectroscopy and hydrogen-deuterium exchange" Arch. Biochem. Biophys. 333, 275-281, 1996. [PDF]

Chang, B.S., Beauvais, R.M., Dong, A., and Carpenter, J.F. "Physical factors affecting the storage stability of freeze-dried interleukin-1 receptor antagonist: glass transition and protein conformation" Arch. Biochem. Biophys. 331, 249-258, 1996. [PDF]

Allison, S.D., Dong, A., and Carpenter, J.F."Counteraction effects of thiocyanate and sucrose on secondary structure and aggregation of chymotrypsinogen during freezing, drying and rehydration" Biophys. J. 71, 2022-2032, 1996. [PDF]

Dong, A., Matssura, J., Allison, S.D., Chrisman, E., Manning, M.C., and Carpenter, J.F. "Infrared and circular dichroism spectroscopic characterization of structural differences in beta-lactoglobulin A and B" Biochemistry 35, 1450-1457, 1996. [PDF]

Kendrick, B.S., Dong, A., Allison, S.D., Manning, M.C., and Carpenter, J.F. "Quantitation of overlap of infrared second derivative spectra to determine structural similarity between proteins" J. Pharm. Sci. 85, 155-158, 1996. [PDF]

Dong, A., Huang, P., and Caughey, W.S. " Redox-dependent changes in beta-sheet and loop structures of Cu,Zn superoxide dismutase in solution as observed by infrared spectroscopy" Arch. Biochem. Biophys. 320, 59-64, 1995. [PDF]

Dong, A., Huang, P., Caughey, B., and Caughey, W.S. "Infrared analysis of ligand- and oxidation-induced conformational changes in hemoglobins and myoglobins" Arch. Biochem. Biophys. 316, 893-898, 1995. [PDF]

Dong, A., Prestrelski, S.J., Allison, S.D., and Carpenter, J.F. "Infrared spectroscopic studies of lyophilization and temperature induced protein aggregation" J. Pharm. Sci. 84, 415-424, 1995.[PDF]

Datta, G., Dong, A., Witt, J., and Tu, A.T. "Biochemical characterization of basilase, a fibrinolytic enzyme from Crotalus basiliscus" Arch. Biochem. Biophys. 317, 365-373, 1995. [PDF]

Hermann, L., Bowler, B.E., Dong, A., and Caughey, W.S. "The effects of hydrophilic to hydrophobic surface mutations on the denatured state of iso-1-cytochrome c: investigation of aliphatic residues" Biochemistry 34, 3040-3047, 1995. [PDF]

Huang, P., Dong, A., and Caughey, W.S. "Effects of dimethylsulfoxide, glycerol, and ethylene glycol on secondary structure of cytochrome c and lysozyme by infrared spectroscopy" J. Pharm. Sci. 84, 387-392, 1995. [PDF]

Dong, A., and Caughey, W.S. "Infrared methods for study of hemoglobin reaction and structures" Methods Enzymol. 232, 139-175, 1994. [PDF]

Dong, A., Nagai, M., Yoneyama, Y. and Caughey, W.S. "Determination of the amounts and oxidation states of hemoglobins M Boston and M Saskatoon in single erythrocytes by infrared microspectroscopy" J. Biol. Chem. 269, 25365-25368, 1994. [PDF]

Bowler, B.E., Dong, A., Caughey, W.S. "Characterization of the guanidine-HCl denaturation state of iso-1-cytochrome c by infrared spectroscopy" Biochemistry 33, 2402-2408, 1994. [PDF]

Dong, A., Caughey, W.S., and Du Clos, T.W. "Effects of calcium, magnesium, and phosphorylcholine on secondary structures of human C-reactive protein and serum amyloid P component observed by infrared spectroscopy" J. Biol. Chem. 269, 6424-6430, 1994. [PDF]

Dong, A., Huang, P., Zhao, X.-J., Sampath, V., and Caughey, W.S. " Characterization of sites occupied by the anesthetic nitrous oxide within proteins by infrared spectroscopy" J. Biol. Chem. 269, 23911-23917, 1994. [PDF]

Bowler, B.E., May, K., Zaragoza, T., York, P., Dong, A., and Caughey, W.S. "Destabilizing effects of replacing a surface lysine of cytochrome c with aromatic amino acids" Biochemistry 32, 183-190, 1993. [PDF]

Bowler, B.E., Dong, A., and Caughey, W.S. "Effects of surface mutations on protein stability: a role for the denatured state." Miami Short Report, Volume 3, Advances in Gene Technology:Protein Engineering and Beyond (Brew, K., et al., Eds.)p.8, 1993. Oxford University Press.

Caughey, W.S., Dong, A., Sampath, V., Yoshikawa, S., and Zhao, X.-J. "Probing heart cytochrome c oxidase structure and function by infrared spectroscopy" J. Bioenerg. Biomembr. 25, 81-91, 1993. [PDF]

Dong, A., Huang, P., and Caughey, W.S. "Redox-dependent changes in beta extended chain and turn structures of cytochrome c in water solution determined by second derivative amide I infrared spectra" Biochemistry 31, 182-189, 1992. [PDF]

Dong, A., Caughey, B., Caughey, W.S., Bhat, K.S., and Coe, J.E. "Secondary structure of the pentraxin female protein in water determined by infrared spectroscopy: effects of calcium and phosphorylcholine" Biochemistry 31, 9364-9370, 1992. [PDF]

Caughey, B., Dong, A., Bhat, K.S., Ernst, D., Hayes, S.F., and Caughey, W.S. "Secondary structure analysis of the scrapie-associated PrP 27-30 in water by infrared spectroscopy" Biochemistry 30, 7672-7682, 1991. [PDF]

Dong, A., Huang, P., and Caughey, W.S. " Protein secondary structures in water from the second derivative amide I infrared spectra"Biochemistry 29, 3303-3308, 1990. [PDF]

Gorga, J.C., Dong, A., Manning, M.C., Woody, R.W., Caughey, W.S., and Strominger, J.L. "Comparison of the secondary structures of human class I and class II major histocompatibility complex antigens by Fourier transform infrared and circular dichroism spectroscopy" Proc. Natl. Acad. Sci. USA 86, 2321-2325, 1989; NATO Life Sci. Ser. A. Protein Structure and Engineering (Jardetzky, O., and Nicolini, C., Eds.) Plenam Press, N.Y. 1989.

Shimada, H., Dong, A., Matsushima-Hibiya, Y., Ishimura, Y., and Caughey, W.S. "Distal His-Arg mutation in bovine myoglobin results in a ligand binding site similar to the abnormal beta site of hemoglobin Zurich (beta 63His-Arg)" Biochem. Biophys. Res. Commun. 158, 110-114, 1989.

Dong, A., Messerschmidt, R.G., Reffner, J.A. and Caughey, W.S. "Infrared spectroscopy of a single cell - the human erythrocyte" Biochem. Biophys. Res. Commun. 156, 752-756, 1988.

Sowa, S., Dong, A., Roos, E.E., and Caughey, W.S. "The anesthetic nitrous oxide affects dioxygen utilization by bovine heart and bean seed mitochondrial particles" Biochem. Biophys. Res. Commun. 144, 643-648, 1987.