We have been fortunate to host several visiting scientists from various parts of the world as we pursue questions about venomous animals and their venoms, and we welcome more research visitors! Their presence in my lab has helped broaden the international perspectives of students working in my lab, and in turn these visitors have been able to take back great life experiences gained in the lab and in Colorado. In addition, we have had great scientific collaborations, most of which are ongoing. Some of these scientists include the following:
Dr. Zamira Golubenko, a senior scientist from the A.S. Sadykov Institute of Bioorganic Chemistry in Tashkent, Uzbekistan, came to my lab to work on Latrodectus spider venoms in 2001 and again in 2005. This work was sponsored by the Civilian Research and Development Foundation and provided support for civilian research projects conducted by scientists of former Soviet Union states. Dr. Golubenko was a delightful colleague and it was a pleasure to have her here at UNC, and we published results of her work conducted at UNC and in Uzbekistan.
Akhunov, A.A., Golubenko, Z., Abdurashidova, N.A., Mustakimova, E.Ch., Ibragimov, F.A. and Mackessy, S.P. 2001. Comparative biochemistry of the physiologically active components of venom, hemolymph and eggs of the karakurt spider (Latrodectus tredecimguttatus). Khimiya Prirodnykh Soedinenii [In Russian]. [Chemistry of Natural Compounds] 37 (6):562-565, November - December, 2001.
Dr. H. Lisle Gibbs, Professor in the Department of Evolution, Ecology, and Organismal Biology at the Ohio State University, visited UNC on several occasions between 2006-2010 to discuss Massasauga Rattlesnakes and evolution of venom phenotypes, as well as attempt to develop a cell-based replacement assay for whole animal LD50s. These successful collaborations with Lisle and his colleagues have resulted in several publications.
Gibbs, H.L. and S.P. Mackessy. 2009. Functional basis of a molecular adaptation: prey-specific toxic effects of venom from Sistrurus rattlesnakes. Toxicon 53(6):672-679.
Sanz, L., H.L.Gibbs, S.P. Mackessy and J.J. Calvete. 2006. Venom proteomes of closely related Sistrurus rattlesnakes with divergent diets. Journal of Proteome Research 5:2098-2112.
Dr. Maria E. Peichoto, then a postdoctoral research scientist from the University of Buenos Aires, Argentina, received a Fulbright grant to work in my lab at UNC in winter 2009-2010, along with her husband, Dr. Flavio L. Tavares (from Brazil), on several projects involving venoms and purified components from rear-fanged “colubrid” snakes. Their short but intense stay resulted in several publications, and we continue to collaborate on projects at present. Part of my sabbatical leave in 2017 will be spent working with them in Foz do Iguaçu (Brazil) and Puerto Iguazú (Argentina), where we will work with venoms of rear-fanged snakes in the vicinity of the fantastically beautiful Iguazú Falls National Parks. Dr. Peichoto is now a research scientist at the INMeT in Puerto Iguazú, and Dr. Tavares is now a Professor at the Federal University for Latin American Integration in Foz do Iguaçu.
Saviola, A.J., M.E. Peichoto, S.P. Mackessy. 2014. Rear-fanged snake venoms: an untapped source of novel compounds and potential drug leads. Toxin Reviews 33(4):185–201.
Peichoto, M.E., F.L. Tavares, M.L. Santoro, S.P. Mackessy. 2012. Venom proteomes of South and North American opisthoglyphous (Colubridae and Dipsadidae) snake species: A preliminary approach to understanding their biological roles. Comparative Biochemistry and Physiology part D 7:361-369
Peichoto, M.E., F.L. Tavares, G. DeKrey, S.P. Mackessy. 2011. A comparative study of the effects of venoms from five rear-fanged snake species on the growth of Leishmania major: identification of a protein with inhibitory activity against the parasite. Toxicon 58:28–34.
Dr. Ashis Mukherjee, a Professor of Molecular Biology and Biotechnology from Tezpur University in Tezpur, Assam, India, stayed in Greeley and worked in our lab at UNC for 1 year in 2011-2012 while supported by a CREST fellowship grant from India. Joining him were his wife Abira and their son Anandan, all three wonderful people who were a pleasure to host. Ashis was incredibly productive, and he worked with venom of the Russell’s Viper (from Pakistan), one of the Big Four venomous snakes of India and South Asia that is responsible for untold human morbidity and mortality. Dr. Mukherjee literally took apart this venom in an effort to uncover useful compounds with potential therapeutic applications and to determine the venom proteome as a part of understanding how to treat some of the more refractive symptoms of bites by this species, including severe coagulopathies and necrosis. Some of the many papers resulting from this work include the following:
Mukherjee, A.K., S. Dutta, B. Kalita, D.K. Jha, P. Deb, S.P. Mackessy. 2016. Structural and functional characterization of complex formation between two Kunitz-type serine protease inhibitors from Russell’s Viper venom. Biochimie 128-129:138-147.
Mukherjee, A.K., B. Kalita, S.P. Mackessy. 2016. A proteomics analysis of Pakistan Daboia russelii russelii venom and assessment of potency of Indian polyvalent and monovalent antivenom. Journal of Proteomics 144:73-86.
Mukherjee AK, AJ Saviola, PD Burns, SP Mackessy. 2015. Apoptosis induction in human breast cancer (MCF-7) cells by a new L-amino acid oxidase (Rusvinoxidase) purified from Daboia russelii russelii venom is independent of its enzymatic activity and does not require caspase-3 activation. Apoptosis 20:1358-1372.
Mukherjee, A.K., S.P. Mackessy. 2013. Biochemical and pharmacological properties of a new thrombin-like serine protease (Russelobin) from the venom of Russell’s Viper (Daboia russelii russelii) and assessment of its therapeutic potential. Biochimica et Biophysica Acta 1830:3476–3488.
Dr. Raquel Sanz-Soler, then a PhD student with Dr. Juan J. Calvete at the Instituto de Biomedicina in Valencia, Spain, spent 4 months in my lab in 2013 to work with expressing RGD disintegrins and comparing their activity with venom-derived disintegrins. Her work was very successful and we anticipate publishing some of her results soon. Dr. Sanz-Soler recently received her PhD and is now living in Germany.